The problem of grippe viruses detecting is very important because of its possible dangerous epidemic and pandemia scale all around the world. There were developed some methods of grippe viruses detecting based on application of spectroscopic and fluorescence technique [1]. All of them couldn't visualize directly the grippe virus modifications, need few days for examination and consist many steps of operation. To avoid the disadvantages the new contact technique based on NLC application was suggested. On glass plate the antibody-viruses sandwich was placed and coated with thin NLC layer of mixture MBBA:EBBA. In the case of antibody-viruses unconformity the complimentary reaction doesn't take place that may be directly observed through polarizing microscope (fig. 1b). In the case of antibody-viruses conformity the complimentary reaction takes place that visualize quite different regular surface structure with crystal-like segments (fig. 1c). The effective results were achieved in usage polyaniline (PA) layer that obtained a good adhesion of antibody material to the glass plate. In some cases the same results were obtained without PA layer. The described LC vision technique has some advantages in comparison with technique based on lyotropic chromonic LCs that is expensive and cannot visualize directly the grippe virus modifications.

It is possible to conclude that new simple and expressive technique based on NLC application to grippe viruses detection is developed. It promises the possibility for direct observation of the surface structures in the case of virus' mutation, for example the bird's flue.

1. Song J., Cheng Q., Zhu S., Stevens R.C. “Smart” materials for biosensing devices: cell-mimicking supramolecular assemblies and colorimetric detection of pathogenic agents // Biomedical Microdevices: 4:3. 2002. P. 213-221. 2. Shiyanovskii S.V., Schneider T., Smalyukh I.I. et al. Real-time detection based on director distortion around growing immune complexes in lyotropic chromonic liquid crystals // Phys. Rev. E 71. 2005. 020702-1-020702-4.

Fig. 1. The structure of antibody drifted on glass plate covered with PA layer for 3 cases: Left – test object in absence of NLC: PA layer is left and PA layer covered with viruses is right from the boundary; Middle – test object with antibody A-viruses B sandwich covered with NLC (left - uncomplimentary reaction) and not covered (right - complimentary reaction); Right - test object with antibody B-viruses B sandwich covered with NLC (left) and not covered (right). The image diameter is 800 nm;